Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin
© Corrales Ureña et al. 2016
Received: 30 November 2015
Accepted: 1 February 2016
Published: 12 February 2016
The formation of nano-scale biofilms on hydrophilic silica surfaces from aqueous polypeptide/polysaccharide mixtures containing laccase and maltodextrin was investigated in situ with quartz crystal microbalance with dissipation monitoring (QCM-D). Surface analysis techniques such as X-ray photoelectron spectroscopy (XPS), optically stimulated electron emission (OSEE) and atomic force microscopy (AFM) were applied for characterizing the resulting layers obtained after periods varying from a few seconds to several hours of contact between the substrate and the biopolymers formulation. The biofilm formation in contact with the aqueous laccase/maltodextrin suspension was studied at pH level 4.75, under conditions close to the isoelectric point of the enzyme. The few nanometers rough biofilms obtained were composed of a laccase/maltodextrin mixture, and their thickness was observed to steadily increase during 4 h of contact with the aqueous mixture of biopolymers. Remarkably, the still adhesive films obtained after 1 h of contact with aqueous polypeptide/polysaccharide mixture resisted a 30 min rinsing with acetate buffer. The biofilms growing process was monitored using OSEE, due to the effected attenuation of the UV-induced electron emission from the SiO2/Si substrate, which was found to be more pronounced than the attenuation of the photoelectrons from the substrate which contribute to the XPS signals. Layers as thin as 1 nm were detected by the OSEE.
The protein adsorption on biomaterials, such as titanium alloys or surfaces of pharmaceutical vials as glass containers, is influenced by complex interactions implying the excipients in the protein based formulations [1–4]. In the case of pristine glass container surfaces the proteins are known to adsorb, lowering their concentration and reducing the availability of therapeutics in solution. Surfactants, polysaccharides and salts are added to pharmaceutical products to stabilize protein formulations and to avoid denaturalization and aggregation [1, 5, 6]. Understanding the biofilm formation upon contact with biopolymer mixture formulations on solid surfaces is essential for the design of a biocompatible material surface that can inhibit or enhance protein adsorption, depending on the respective application .
The interaction of proteins or polypeptides with molecules such as polysaccharides is complex; aggregation and association processes observed in solution depend on the conditions of the media and on the ongoing surface-related processes such as cooperative adsorption, displacement and aggregation. In addition, bulk and surface interactions need to be considered [8, 9]. In general, surface modification with mixtures containing surfactants and proteins have been extensively studied in solid/liquid interfaces  as well as the behavior of mixtures comprising proteins and polysaccharides at liquid/liquid interfaces as in water/oil emulsions .
This study is focused on the interaction of an aqueous suspension of laccase and a polyssacharide with a hydrophilic surface. The polysaccharide maltodextrin was added as a stabilizing and a lyophilizating agent to laccase enzyme formulation . The involved mode of action was suggested to be based on hydrogen bonding, with the hydroxyl groups of maltodextrin having a role firstly as a stabilizing agent in the solution , and secondly by replacing the water molecules lost upon drying the protein material . Biofilms produced by the thus resulting protein/polysaccharide complexes are used in industry for production of food packaging, sutures, wound dressings or for protein-carbohydrates based adhesives [17, 18], mainly because of their favorable cohesive and adhesive properties as well as their biodegradability and environmental safety  . On the other hand, polysaccharides, such as dextrans, are used to avoid protein adsorption on biomedical devices, such as contact lenses and artificial blood vessels . The anti-adhesion properties are related to the reduction of the interfacial energy, the large exclusion volume or influences on the system entropy impacted by the heavily solvated, the neutral and randomly oriented polymer chain and the sterical impediment, being similar to the effect of polyethylene glycol films [21–24]. However, adhesive properties also depend on the surface roughness of the adsorbent, the protein carbohydrate combination  or factors such as pH and ionic strength in the liquid phase [26, 27]. Therefore, the adsorption behavior is characteristic for each singular protein, and computer-based simulation may permit recommending trends or development directions . For example, albumin adsorbs on both hydrophobic and hydrophilic surfaces, even if the polarity of charges on the protein and the surface is the same, whereas ribonuclease is reported to adsorb on hydrophobic surfaces under all conditions of charge, but to adsorb on hydrophilic surfaces only if the interaction is electrostatically favored .
In the present study, the layer formation from polypeptide/polysaccharide mixtures containing laccase and maltodextrin on hydrophilic Si/SiO2 surfaces was investigated. The biofilm formation in contact with the aqueous laccase/maltodextrin suspension was studied with a pH value of 4.75, under conditions close to the isoelectric point of the enzyme, where a low electrostatic repulsion between protein molecules and neighboring protein or polysaccharide molecules may be expected. Therefore, in a pH where the enzyme is stable  and their higher catalytic activity .
Quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray photoelectron spectroscopy (XPS), optically stimulated electron emission (OSEE) and atomic force microscopy (AFM) were applied for characterizing the adsorptive growth of the biofilm on the surface.
Laccase mixture suspension
Acetic acid and sodium acetate were used as grade reagents (Sigma-Aldrich). A laccase mixture suspension (concentration 0.1 mg/ml) was prepared with laccase from a Trametes versicolor/maltodextrin powder mixture (ASA Spezialenzyme GmbH, Wolfenbüttel, Germany) in 0.2 M aqueous acetate buffer, pH value 4.75. The suspension was prepared using deionized water and aseptic conditions at 25 °C. These same preparation conditions were used for the QCM-D experiment and for the preparation of SiO2/Si substrates.
Preparation of laccase/silica samples
Silicon wafer with a native oxide layer SiO2/Si (100) were used as a substrate. The laccase mixture suspension was pipetted onto the substrate surface. After the respective contact time (with times varying between 10 s and 4 h), the surface was rinsed gently for some seconds with deionized water, followed by blowing with air. Finally, the samples were allowed to dry and were stored under environmental conditions at room temperature.
Quartz crystal microbalance with dissipation monitoring (QCM-D E4, Q-Sense, Sweden) was to evaluate the adsorption from aqueous laccase mixture suspension on silica coated QCM-D sensors. The flow rate was 100 µL/min and the temperature was maintained constant at 25 °C. The sensors were mounted in the flow modules and the system was equilibrated with buffer for 30 min to generate a stable baseline. Then, the aqueous laccase mixture suspension was flushed over the silica crystals. The changes in the frequency (f), related to the attached mass (including coupled water), and the changes in dissipation (D), related to the frictional (viscous) properties of the adsorbed laccase layer, were recorded using QSoft™ control software (Q-Sense, Sweden). More details of the experimental procedure was described by Corrales et al. .
X-ray photoelectron spectroscopy
XPS spectra were taken using a Kratos Ultra facility and applying the following acquisition parameters: base pressure: 4*10−8 Pa, sample neutralization applying low energy electrons (<5 eV), hybrid mode (electrostatic and magnetic lenses are used), take off angle of electrons 0°, pass energy 20 eV (or, respectively, 40 eV in the case of most N1 s spectra) in high resolution spectra and 160 eV in survey spectra, excitation of photoelectrons by monochromatic Al Kα radiation. The analysis area was elliptically shaped with main axes of 300 x 700 µm. The thus obtained information depth is approximately 10−8 m.
Atomic Force Microscopy AFM
The sample topography and local material properties were approached using a scanning force microscope (SFM) (Digital Instruments Nanoscope III multimode SPM) operated in the ‘tapping mode’ in air, using the scanner with ser. no. 6965JVH and Nanoscope software (Version 5.31R1) for acquiring height and phase images. Si Sensors (model Veeco OTESPA made from 1 Ohm Silicon; back side of the cantilever covered with 50 nm Al coating) with resonance frequencies of 12–103 kHz were used. For characterizing structures of adsorbates, among other criteria, height differences were evaluated. In detail, the particle height was obtained by evaluating the vertical distance between the top of a particle and the slightly rough base area below the base-line separated particles. The root mean square (RMS) roughness was obtained calculating the average of three different areas using the software Nanoscope Analysis Bruker.
Optically stimulated electron emission (OSEE)
For the OSEE experiments a surface quality monitor SQM300 (Photo Emission Tech., Inc. (PET), USA) was used. Under ambient conditions the sample surface is exposed to ultra-violet light from a mercury vapor lamp (emission maxima 4.9 and 6.7 eV.). The device contains a solid state electrometer capable of measuring emitted photoelectrons from the tested samples. Those photoelectrons flow through a collector (adjusted at +40 V), generating a current and its intensity is denoted as the OSEE intensity. The device features an earthed moving table on which the samples are scanned in orthogonal directions within a few millimeters distance to the UV lamp. The OSEE signal values presented in this work were obtained by calculating the average of the OSEE intensities from the center of the samples.
Experimental results and discussions
In this chapter, first the in situ experiments focusing on the adsorption from the aqueous biopolymer formulation on silica surfaces are detailed. Subsequently, the ex situ characterization of the biofilms by spectroscopic and microscopic techniques will be reported.
Following the layer formation in situ by QCM-D, Fig. 1 shows the mass uptake related to the changes in oscillation frequency of the silica substrates related to the absorbates getting attached from the laccase—maltodextrin suspension. As described in the experimental section, firstly the pure acetate buffer solution was flushed over the crystal surface (baseline), stage I, followed by a contact-period with the laccase-maltodextrin suspension, stage II. In stage II a decrease of the frequency indicates mass adsorption. A steady state was not achieved after one and a half hours of contact with the suspension. The slow decrease in the frequency was observed in the early stages of biofilm growth on the silica substrate and, later on, on top of the already formed biofilm as suggested by the results of AFM investigations presented in the next section. Such small growth rate might be considered an indicative of a low affinity of the biopolymers for adsorption to the surface. A steady state, stage III, was not achieved during the contact time, as it will be presented in further studies by the authors. In stage IV and V the surface was flushed with the buffer for 30 min and only low desorption of the biofilm in the early stage IV was detected. The insignificant extent of desorption of the surface is related to a surprisingly stable layer formation.
The mass adsorbed was calculated using Sauerbrey equation due to the low changes in dissipation  (data not shown). The layer thickness of 2.3 nm reached after 74 min of contact was calculated having as reference the density of a hydrated protein layer of 1.3 g/cm3 , the experimentally observed mass value of 320 ng/cm2 and assuming an homogeneous surface coverage.
Atomic force microscopy (AFM)
Evaluation of the AFM results obtained when increasing the contact time between SiO2/Si substrates and aqueous laccase/maltodextrin mixture suspensions
Root mean square (RMS) roughness
Particle height (nm)
0.4 ± 0.1 nm
1.3 ± 0.1 nm
2.6 ± 1.4
3.9 ± 1.5
1.7 ± 0.1 nm
2.8 ± 0.2
6.7 ± 0.7
2.6 ± 0.3 nm
6.2 ± 0.7
2.8 ± 0.2 nm
8.6 ± 0.7
These observations coincide with the increase of the RMS roughness values (RMS) of the biofilms with increasing contact time. This finding is associated with biopolymer adsorption.
Elemental surface composition after contact of SiO2/Si substrates with aqueous laccase mixture suspension
Time in contact
[Si] (at %)
[O] (at %)
[C] (at %)
[N] (at %)
[Na] (at %)
Purified laccase powder
Laccase-maltodextrin powder mixture
The layer thickness of the dry film may be calculated based on the XPS results obtained if a geometric model for the adsorbate structure is assumed. Following the data presented by Seah and Fritz, the inelastic mean free path (IMFP) of Si2p electrons in an organic adsorbate layer is 3.2 nm. Assuming a flat, smooth, homogeneous organic layer to be formed after 4 h, a layer thickness of 1.2 nm might be expected. However, such thickness is lower than the roughness of the layer as observed by AFM measurements. Thus, the layer thickness will be underestimated.
Conclusions and outlook
This work presented results obtained with in situ and ex situ surface analyses illustrate the biofilm formation on silica-based surfaces from a biopolymer mixture suspension composed by a glycoprotein and a polysaccharide. The protein and the polysaccharide are revealed to coadsorb on the surface forming a mixed nanolayer exhibiting particulate adsorbates. Concerning projected applications of the findings, the laccase/maltodextrin mixture suspension might be used for the bio-functionalization of technical surfaces, and for tuning the adhesive properties of these surfaces. On the other hand, glass surface modifications may be tested with respect to their effectivity using the contact with standardized laccase-maltodextrin mixtures.
YCU planned and performed AFM investigations, drafted the manuscript; LG conducted the measurements and interpretation of the QCM-D data. MV planned OSEE experiments under the surpervision of PLN and performed sample preparation; PLN performed OSEE and XPS measurements and evaluated them; PNL provided information about technological boundary conditions of the surface pre-treatment and technological challenges; WL participated in the identification of the research topic, the conception of the investigations and drafting the manuscript; KR participated in the identification of the research topic, the conception of the investigations and drafting the manuscript. All authors read and approved the final manuscript.
The authors thank to Science without Borders (Ciência sem Fronteiras, proc: 88888.015774/2013-00,88888.020073/2013-00), Consejo Nacional para Investigaciones Científicas y Tecnológicas de Costa Rica (CONICIT) and FAPESP 2013/07296-2 for the funding. We heartily thank to Prof. Dr. Bernd Mayer, Dr. Ingo Grunwald and to Prof. Dr. Andreas Hartwig for the support.
The authors declare that they have no competing interests.
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